Acatris Leads The Way In Validating Soy Isoflavone Testing Methods

Source: Acatris Holding B.V., The Netherlands

Giessen, The Netherlands – Acatris based in The Netherlands, is releasing the results of its ring test that compared soy isoflavone testing methods of various universities, commercial, and industry laboratories throughout Europe, United States, Canada, Japan, and Australia. Currently there is considerable variation among measurement techniques used to establish isoflavone levels and the unfortunate outcome of this problem is that comparing isoflavone levels based on the stated value of food and supplements labels results in an unfair analysis. In the end, the consumer is left with the proverbial problem of comparing apples to oranges rather than apples to apples.

Acatris contracted with TNO Nutrition and Food Research to perform a ring test with a total of 22 laboratories from around the world. The laboratories were asked to analyze the level of isoflavones in duplicate samples of three soy preparations using their laboratories current method of analysis as well as to submit their method of analysis for further statistical comparisons. Twenty laboratories used an HPLC method, one laboratory used an enzyme-linked immunosorbent assay (ELISA), and the last used capillary electrophoresis. Although the results were positive for the laboratories’ ability to duplicate the same results twice, the variation between the laboratories’ results were statistically unacceptable. Reasons for this included variations in the method of analysis such as differences in the extraction conditions, differences in the molar extinction coefficients used to calculate isoflavone values, and an unknown purity of the standards used to compare the soy isoflavones. This test confirmed the variance that exists in the much-debated topic of isoflavone testing methods.

It should be noted that last December the Association of Official Analytical Communities (AOAC) developed a soy isoflavone testing method for food. Major issues going forward appear to be whether or not this method will be adopted, and if so, by whom? This method does not address measuring isoflavone levels in biological systems so it remains to be seen whether or not soy researchers will adopt it or continue to use their own various methods. Acatris continues to search for opportunities to champion a uniform method of soy isoflavone analysis.

For more information please contact us at Acatris Holding B.V, Marian Verbruggen, Director R&D or Petra de Wit, Marketing Communications Manager, tel. +31 183 446 445, or [email protected] and [email protected]

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