Roche's xCELLigence RTCA System is being used in the European Union's SEURAT (Safety Evaluation Ultimately Replacing Animal Testing) initiative to run five years from 2011 to 2016. As of 2013, animal experiments are no longer permitted for use in toxicology testing in the cosmetic industry. Significant progress has been made in recent years to develop alternatives to in vivo toxicity tests for assessing human safety of new products and their ingredients. At present, it is however not possible to completely replace animal testing. This is especially true when testing the effects of repeated doses over longer periods of time.
A SEURAT-sponsored research team comprising Paul Jennings at the Department of Physiology and Medical Physics, Innsbruck Medical University (Austria), and Alex Seiler and Manfred Watzele at Roche Penzberg (Germany), used continuous real-time impedance measurements with the xCELLigence System to investigate renal epithelial monolayer formation and barrier function. To test repeated dose effects, a human renal proximal tubule cell line (RPTEC/TERT1, Evercyte GmbH Vienna) was cultured on electronic sensor-containing E-Plates 96. Cell impedance was monitored over several weeks in culture, and the effect of nephrotoxin treatment on cell impedance was investigated. Findings showed that the three different nephrotoxins initially increased impedance values as epithelial cell morphology was altered, followed by a decrease in impedance due to cell death.
The xCELLigence System was shown to be a sensitive, non-invasive tool for monitoring repeated dose effects on epithelial cell function and cytotoxicity. Unlike the more laborious transepithelial electrical resistance (TEER) assay, this in vitro assay is a simple, automated, non-invasive, functional readout with very high temporal resolution. Assays such as this will we be beneficial in efforts to replace animal testing for long-term repeat dose regimes.
Headquartered in Basel, Switzerland, Roche is a leader in research-focused healthcare with combined strengths in pharmaceuticals and diagnostics. Roche is the world's largest biotech company with truly differentiated medicines in oncology, virology, inflammation, metabolism and CNS. Roche is also the world leader in in-vitro diagnostics, tissue-based cancer diagnostics and a pioneer in diabetes management. Roche's personalized healthcare strategy aims at providing medicines and diagnostic tools that enable tangible improvements in the health, quality of life and survival of patients. In 2011, Roche had over 80,000 employees worldwide and invested over 8 billion Swiss francs in R&D. The Group posted sales of 42.5 billion Swiss francs. Genentech, United States, is a wholly owned member of the Roche Group. Roche has a majority stake in Chugai Pharmaceutical, Japan. For more information: http://www.roche.com.
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