This study determined total flavonoid, procyanidin, anthocyanin and polyphenol content from chokeberry extracts, evaluated the antioxidant activity of the extracts with several assays, and studied the effects of the anthocyanin fraction against a human cancer cell line. The researchers report that antitumor effects appeared to manifest via a pro-oxidant effect within the exposed tumor cells. While the antitumor and antioxidant activities may correlate with chokecherry anthocyanin constituents, beneficial health effects in the body from consumption of chokeberry extracts likely occur by different biochemical mechanisms.
Journal of Medicinal Food
June 25, 2012;DOI:10.1089/jmf.2011.0246.
“Antioxidant Activities of Chokeberry Extracts and the Cytotoxic Action of Their Anthocyanin Fraction on HeLa Human Cervical Tumor Cells”
The present study evaluates the antioxidant activity of two Aronia melanocarpa cultivars-Viking and Aron-and of Aronia prunifolia hybrid in relationship with their phytochemical composition regarding the contents of total phenolics, flavonoids, procyanidins, and monomeric anthocyanins. The antioxidant capacity of the mentioned extracts of chokeberries was evaluated through five complementary assays: 2.2 azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), H2O2 scavenging potential, oxygen radical absorbance capacity, ferric reducing antioxidant power, and cupric ion reducing antioxidant capacity. A. prunifolia hybrid was found to have the highest antioxidant activity and to be the richest in polyphenols, procyanidins, and anthocyanins compared with the A. melanocarpa cultivars. A good correlation was observed between antioxidant activity and total procyanidin and anthocyanin content. Cyanidin glycosides inhibited HeLa human cervical tumor cell proliferation and increased generation of reactive oxygen species after 48 h of treatment, suggesting that they could be responsible for the antiproliferative activity. These results may be significant for industry concerning food quality and disease prevention.